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Figure 3 | Plant Methods

Figure 3

From: Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS

Figure 3

Activity of transgenic (A) and endogenous (B) GUS in Arabidopsis leaves as measured experimentally and corrected for the estimated inhibitory capacity of the extracts. Plots are inferred from data reported in the table annexed to each graph. Extracts are obtained from transformed plants in (A), and wild type plants in (B). Vm and Vc indicate the measured and corrected reaction rates, respectively; C indicates the relative extract concentration, calculated by normalizing the undiluted extract to the unit. As the inhibitor and the enzyme are components of the extract, their relative concentrations coincide with the relative extract concentration. Transgenic and endogenous GUS activity have been measured at different extract concentrations: values (Vm; λ) are the means of triplicate measurements ± standard variation. Inhibitory capacity against commercial E. coli GUS was assessed for each replicate and is reported as percentage of inhibition (I%) as the mean value ± standard variation (refer to Materials and Methods for the detailed procedure followed to produce the data). The estimated percentage of inhibition against E. coli GUS was applied to correct the respective measured reaction rates and the obtained values are reported (Vc; ν). In (A), the trend line interpolates the corrected data, whereas in (B) it simply represents the linearity between extract dilution and activity expected in the absence of inhibitors. Reaction rates are expressed as nanomoles in (A) and picomoles in (B) of MU produced per minute per milligram of protein of the undiluted extract. Left y axis refers to the measured values (Vm), right to the corrected values (Vc).

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