Skip to main content
Figure 2 | Plant Methods

Figure 2

From: Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS

Figure 2

Type of inhibition in Arabidopsis (A) and tobacco (B) leaf extracts as inferred from the Cornish-Bowden plot. Enzymatic activity of commercial E. coli GUS has been measured in the extraction buffer and in the presence of plant extract at different dilutions (1:10, 1:20, 1:50, 1:150 in A; 1:10, 1:15, 1:50 in B), as well as at different substrate concentrations, i.e., 0.5 (υ), 1 (ν), and 2 (λ) mM MUG for Arabidopsis; and 0.3 (υ), 0.6 (ν), and 1 (λ) mM MUG for tobacco. Reaction rates are were calculated as nanomoles of MU produced per minute per microliter of the commercial GUS mother solution added. S/V indicates the ratio between substrate concentration and reaction rate; i indicates the relative inhibitor concentration present in the sample: it has been obtained by normalizing the inhibitor concentration present in the plant extract at the different dilutions to that in the undiluted extract. It corresponds to the reciprocal of the plant extract dilution factor.

Back to article page

Plant Methods

ISSN: 1746-4811