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Table 1 List of standards used for matrix free and matrix adapted calibration.

From: A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS

     Calibration2 Calibration3
Number Substance [M+H] + Retention
time [min]
Linearity
(r) §
LOD
[pg/μL]
Linearity
(r) §
LOD
[pg/μL]
1 13C6-jasmonic acid-isoleucine (13C6-JA-Ile) 421 6.86 1.00 20.60 0.99 116
2 Jasmonic acid-isoleucine (JA-Ile) 415 6.86 1.00 20.80 0.99 117
3 2H4-salicylic acid (2H4-SA) 234 8.77 0.98 24.40 0.98 167
4 Salicylic acid (SA) 230 8.77 0.97 24.80 0.98 165
5 2H6-abscisic acid (2H6-ABA) 362 9.01 0.98 19.40 0.98 163
6 Abscisic acid (ABA) 356 9.02 0.99 18.70 0.98 165
7 Indole-3-carboxylic acid (ICA) 253 9.08 0.93 41.30 0.91 355
8 Royal jelly acid (Tr IS) 278 9.26 0.99 12.00 0.98 181
9 Cinnamic acid (CA) 240 9.34 0.99 23.90 0.97 203
10 Jasmonic acid (JA) 302 9.43 0.98 19.60 0.98 155
11 Traumatol 306 9.85 0.99 12.90 0.98 174
12 Indole-3-acetic acid (IAA) 267 9.94 0.98 19.30 0.99 297
13 Traumatin 304 9.96 1.00 5.30 0.98 168
14 9,10-2H2-dihydro-jasmonic acid (D2-JA) 306 10.13 1.00 11.30 0.98 180
15 Traumatic acid 320 10.15 0.99 11.20 0.96 250
16 Hexadecatrieonic acid (16:3) 342 10.29 0.99 17.00 0.99 132
17 (9 S, 13 S)-12-oxo-phytodienoic acid (OPDA) 384 10.89 1.00 10.30 0.98 147
18 Hexadecadienoic acid (16:2) 344 10.93 0.99 14.90 0.98 147
19 Gibberellin A3 (GA3) 438 11.27 0.93 42.30 0.96 246
20 2H2-OPC 8:0 388 11.59 0.94 *1 0.96 *1
21 Linolenic acid (18:3) 370 12.83 1.00 7.80 1.00 70
22 Hexadecenoic acid (16:1) 346 13.20 0.99 19.00 0.99 95
23 Heptadecenoic acid (17:1) 360 13.78 1.00 4.10 0.97 195
24 Linoleic acid (18:2) 372 14.22 0.97 8.60 0.96 123
25 Hexadecanoic acid (16:0) 348 14.53 1.00 5.20 0.99 114
26 Octadecenoic acid (18:1) 374 14.86 0.99 14.70 0.99 94
27 Heptadecanoic acid (17:0) 362 16.09 1.00 7.50 0.99 119
28 Stearic acid (18:0) 376 17.59 1.00 7.90 0.99 117
  1. *1 Amount of standard unknown.
  2. 2 Matrix free calibration
  3. 3 Matrix adapted calibration
  4. §Standard calibration curves were generated by injecting 10 μL of 100, 250, 375, 500, 750, 1000 pg/μL of the different standards (n = 3).