Plasmids encoding a variety of fluorescent proteins may be successfully delivered to protoplasts. Protoplasts transiently transfected with (A) YFP plasma membrane marker, the full length of AtPIP2A, a plasma membrane aquaporin, ABRC stock no.: CD3-1005, (B) CFP (ABRC stock no.: CD3-953), and (C) a YFP-tagged ER marker (ABRC stock no.: CD3-957), the signal peptide of AtWAK2 (Arabidopsis thaliana wall-associated kinase 2) at the N-terminus and the ER retention signal at C-terminus of the fusion protein . Transfections used the PEG-mediated method. Bar = 5 μm.