Characteristic features of organelle markers in mesophyll-derived protoplasts of stably transformed lines of Arabidopsis. (A) YFP vacuole marker: γ-TIP, an aquaporin of the vacuolar membrane. ABRC stock no.: CS16258. (B) GFP plastid marker: the targeting sequence (first 79 aa) of the small subunit of tobacco Rubisco fused with GFP. ABRC stock no.: CS16266. (C) YFP mitochondria marker: first 29 amino acids of yeast cytochrome c oxidase IV fused with YFP. ABRC stock no.: CS16264. (D) CFP peroxisome marker: cytoplasmic tail and transmembrane domain of soybean -1,2-mannosidase I fused with CFP . ABRC stock no.: CS16259. Bar = 5 μm.