Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Table 2 Tomato TILLING results overview.

Product	Size (Kb)	Screened families	Mutations identified	Mutation detection rate (n mutations/1000 kb)	Mutation density (1/n kb)
SNP Markers	1.467	2300	5	1.48	675
*ARF7*	0.894	5000	5	1.12	894
*ProDH M3*	0.788	6700	8	1.52	660
*ProDH M2*	0.788	8025	12	1.90	527
*PSY*	0.804	8025	8	1.24	807
*Sus2*	0.812	8025	6	0.92	1086
All products	0.8514	38075	44	1.36	737
	(weighted average)	(independent amplicons)

Mutation frequencies on the tomato genome were calculated as follow: ((total size of amplicons) × (total number of screened lines))/(number of identified mutations). The average mutation frequency was calculated as: ((weighted average amplicon size) × (total number of independent amplicons))/(total number of mutations).

ISSN: 1746-4811