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Figure 5 | Plant Methods

Figure 5

From: Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Figure 5

PARSESNP output for the ProDH gene following mutation identification in both M2 and M3 mutagenised populations. (A) PARSESNP graphical positioning of identified SNPs. The orange box represents the ProDH coding sequence. The black triangles represent the position of the mutations. Black stripes represent the PCR fragments that were analysed with HRM. (B) Following high-throughput mutation screening, ProDH putative mutant families were sequenced. G to A and C to T transitions are identified as double peaks. In total 19 mutations were identified. Here four chromatograms displaying mutations are shown as examples, they correspond to the green circled arrow heads in (A).

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Plant Methods

ISSN: 1746-4811