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Figure 3 | Plant Methods

Figure 3

From: Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations

Figure 3

Output peaks from CSCE screen. (A) Negative control peak, represents a pool not containing any mutation, all the fragments migrate through the capillary at the same speed. (B) Positive control peak represents a pool containing DNA isolated from S. pimpenellifolium seeds. The fragments forming heteroduplexes have a different motility through the CAP polymer than the majority of the other products. The homoduplexes in this example run faster. C, D & E; Examples of peak patterns different from either the positive or the negative controls and therefore identified as mutant, one line in each of these pools contain a mutation. Direct sequencing confirmed the results obtained with CSCE. (A) C2_At4g11570 negative control; (B) C2_At4g11570 Positive control; (C) C2_At4g11570 mutant; (D) Expansin1 mutant (E) Second Expansin1 mutant

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