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Table 1 Morphological parameters of isolated nuclei before and after FISH treatments compared to untreated nuclei from leaf cryosections.

From: 3D fluorescent in situ hybridization using Arabidopsis leaf cryosections and isolated nuclei

  N Diameter (μm) Compactness NZ Flatness
Untreated 65 5.99 ± 0.72 0.79 ± 0.07 21 1.54 ± 0.36
Before FISH 77 5.85 ± 0.85 (p = 0.25) 0.77 ± 0.11 (p = 0.72) 71 1.56 ± 0.40 (p = 0.87)
After FISH 64 5.79 ± 0.83 (p = 0.14) 0.74 ± 0.14 (p = 0.36) 48 1.79 ± 0.53 (p = 0.08)
  1. N: number of analyzed nuclei; NZ: number of nuclei with the shortest axis aligned with the Z-axis and used to compute flatness. The p-values of the pair wise comparisons between isolated nuclei and untreated cryosection nuclei are given in parenthesis. ±: Standard error. Untreated: cryosections.