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Table 3 Amplicon sizes used for heterozygote identification from F2 individuals

From: High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination

Primer combination

Polymorphic amplicon

Normalised to monomorphic pair

  

nt

nt

6FAMPPT/PPT/Taq+CA

p1

261

252 and 270

6FAMPPT/PPT/Taq+CA

p2

274

252 and 270

6FAMPPT/PPT/Taq+CA

p3

307

252 and 270

HEXPPT/PPT/Taq+AT

p4

396

177 and 469

HEXPPT/PPT/Taq+AT

p5

193

177 and 469