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Table 2 Mean peak area ratios in F1 zygosity tests

From: High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination

 

Homozygote

Heterozygote

     

Normalised to m1/m2

Normalised to m3/m4

Peak

m1

m2

m3

m4

p1

p2

p3

p1

p2

p3

Size (nt)

287

289

448

470

295

323

324

295

323

324

aw/a†

1.0

(0.03)

1.0 (0.03)

0.84 (0.02)

1.23 (0.03)

  

0.55 (0.07)

  

0.44 (0.03)

aw/w†

0.98 (0.04)

1.01 (0.03)

1.08 (0.03)

0.91 (0.03)

0.51 (0.03)

0.53 (0.08)

 

0.6 (0.03)

0.62 (0.07)

 

wa/a†

0.99 (0.03)

1.02 (0.04)

0.83 (0.04)

1.25 (0.06)

  

0.53 (0.03)

  

0.43 (0.02)

wa/w†

0.97 (0.04)

1.03 (0.04)

1.06 (0.06)

0.95 (0.05)

0.46 (0.03)

0.55 (0.06)

 

0.55 (0.07)

0.66 (0.04)

 

Mean (SD) of 48

 

1.04

(0.04)

 

1.02

(0.16)

 

Mean (SD) of 36

0.52

(0.06)

 

Mean

(SD) of 36

0.55

(0.1)

   

Mean (SD) of 96

1.01

(0.11)

    

Mean

(SD) of 72

0.54

(0.08)

  1. † a = Avola; w = Waverex; aw = Avola × Waverex; wa = Waverex × Avola
  2. A blank cell indicates no value as the peak was absent. The raw peak area values from the F1 data in Additional file 1 were first normalised with respect to the sum of the peak areas of the monomorphic pairs m1/m2 and m3/m4 and then the ratio of these normalised values was determined with an expected mean of 1 for the homozygote and 0.5 for the heterozygote. Values for each class show the mean from six F1s with the SD in brackets. Collective means and SD for both the homozygote and heterozygote classes can be seen in the bottom two rows [see Additional file 1 for all data input to this Table].
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Plant Methods

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