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Figure 2 | Plant Methods

Figure 2

From: High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination

Figure 2

33P band and fluorescent peak pattern comparison. Fluorescent peak patterns with the triplex primer combination 6FAMPPT/PPT/Taq+TG, showing individual electropherograms for specific amplicons, A 1039 – 1060 nt for JI399, C 802 and 1039 – 1058 nt for JI281; the horizontal axis represents nt, and the vertical axis represents relative fluorescent units (RFU) [20]. The corresponding radio-labelled reactions with 33P PPT/Taq+TG for both accessions, shown replicated in B, covers a partial gel image from 247 to ca. 1100 nt. The band sizes (nt) were determined by virtue of their pattern of occurrence from the 33P gels and their corresponding fluorescent peak assessed with GeneMarker Version 1.6 software.

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