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Figure 1 | Plant Methods

Figure 1

From: High-throughput retrotransposon-based fluorescent markers: improved information content and allele discrimination

Figure 1

Schematic of a PDR1 insertion in the pea genome and template for PCR. A. The PDR1 insertion (grey area with black blocked termini) within genomic DNA (thick black horizontal lines), with the Taq I sites (thin vertical lines) shown. The PDRI element first sequenced (X66399.em.pl) has five known Taq I sites (vertical lines). A sixth Taq 1 site external to the element is shown. B. The distance to an external site differs from one PDR1 insertion site to another; the Taq adapter is shown (chequered line) at this location. PCR is carried out with the labelled PPT (polypurine tract) – specific primer of the element, which is adjacent to the LTR (long terminal repeat – black blocked termini with chevron), and the Taq adapter primer with two bases of selection on the 3'-end. A range of amplicon sizes are obtained dependent on the position of Taq I sites 3' to the element insertion within the pea genome.

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