Figure 2

SAP-based genotyping of three different mutant alleles. (A) WT LUG and MT lug-16 genotypes were identified by the positive amplification of a 401 bp band when the WT (LUG) primer and the lug-16 MT primer amplify their WT and MT target template DNA, respectively. Similarly, WT (LUH) and MT luh-1 genotypes were identified when a 456 bp PCR fragment was amplified with respective primers. (B) Presence of WT (LUG) and MT lug-3 template DNA correlates with the amplification of a 301 bp PCR band using respective WT (LUG) and MT lug-3 primers. A heterozygote (F1 progeny of a cross between wild type and lug-3) correlates with the positive PCR amplifications with both WT (LUG) or MT lug-3 primers.