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Figure 4 | Plant Methods

Figure 4

From: Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings

Figure 4

Detection of nuclei, plastids, and mitochondria in a Kaleidocell root. (A) A maximum projection image of a vertical series of scanning confocal micrographs of a root of a Kaleidocell seedling. The root grew along the x-axis. A total of 17 epidermal and 8 cortical cells (green lines) were identified. Cell boarders at the ends of the x-axis (top and bottom of the image) were beyond the scanning area. Within a total of the 25 cells, 11 nuclei (blue spots), 255 plastids (red spots), and 10,158 mitochondria (green spots) were identified. A white thin horizontal line in the middle of the image indicates a point where a cut-through image was generated. (B) Cut-through image of the stacked image. Image contrast was adjusted to enable clear visualization of the cell borders. Scale bars = 10 μm.

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