High quality metabolomic data for Chlamydomonas reinhardtii

Table 2 Technical error rates for metabolite profiling of Chlamydomonas reinhardtii cells.

Homogenization	Solvent system	Identified metabolites	All peaks
glass beads	MCW	30%	39%
glass beads	WiPM	26%	34%
steel ball	MCW study1	16%	22%
steel ball	MCW study2	18%	23%
steel ball	WiPM study1	21%	30%
steel ball	WiPM study2	25%	38%
steel ball	MeOH	30%	40%
steel ball	WiPA	28%	38%
steel ball	MCW (10/3/1)	30%	37%

The median precision (as relative coefficient of variance) is given for the 80 compounds identified by BinBase, resp. over all 334 peaks that were annotated metabolic signals by BinBase. Study 1 comprised the comparison of grinding methods, study 2 compared different solvents. Abbreviation of solvents as given in figures 3–5.

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