Study 3. Application of the optimized method for metabolite profiling of C. reinhardtii cells on different time points after inoculation of fresh cultures at 5 × 10^5 cells/mL ; N = 6. Green: 1 h, Red: 4 h, Blue: 10 h, Black: 22 h after inocculation. Upper panel: discrimination of metabolic phenotypes by partial least square analysis (PLS); Mid and lower panels: differential control of metabolism for selected compounds. One-way breakdown analysis of variance by box-whisker plots.