Study 1 on C. reinhardtii homogenization and extraction protocols, N = 9. Black: steel ball disruption + extraction by water/isopropanol/acetonitrile (WiPA). Blue: steel ball + methanol/chloroform/water (MCW). Green: glass bead disruption + WiPA. Orange: glass bead disruption + MCW. Upper panel: Supervised partial least square analysis (PLS); red circle: 3 × S.E. distance. Lower panel: Univariate one-way analysis of variance; units: normalized peak heights. Boxes show 1 × S.E, whiskers indicate 1.96 × S.E. data distribution.