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Table 2 Oligonucleotide combinations used for amplification of NbPDS VIGS inserts

From: Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus

Construct name Primer combination
NbPDS (2046 bp) NbPDSfor1 × NbPDSrev1
NbPDS (1661 bp) NbPDSfor2 × NbPDSrev1
NbPDS (1304 bp) NbPDSfor3 × NbPDSrev1
NbPDS (959 bp) NbPDSfor4 × NbPDSrev1
NbPDS (610 bp) NbPDSfor5 × NbPDSrev1
NbPDS (257 bp) NbPDSfor6 × NbPDSrev1
NbPDS (345 bp) NbPDSfor3 × NbPDSrev2
NbPDS (192 bp) NbPDSfor7 × NbPDSrev3
NbPDS (103 bp) NbPDSfor7 × NbPDSrev4
NbPDS (54 bp) NbPDSfor8 × NbPDSrev5
NbPDS (385 bp) NbPDSfor1 × NbPDSrev6
NbPDS (357 bp) NbPDSfor2 × NbPDSrev7
NbPDS (349 bp) NbPDSfor4 × NbPDSrev8
NbPDS (353 bp) NbPDSfor5 × NbPDSrev9
NbPDS (2046 bp + polyA) NbPDSfor1 × NbPDSrev6
NbPDS (1661 bp + polyA) NbPDSfor2 × NbPDSrev6
NbPDS (1304 bp + polyA) NbPDSfor3 × NbPDSrev6
NbPDS (959 bp + polyA) NbPDSfor4 × NbPDSrev6
NbPDS (610 bp + polyA) NbPDSfor5 × NbPDSrev6
NbPDS (257 bp + polyA) NbPDSfor6 × NbPDSrev6
NbPDS (345 bp + polyA) NbPDSfor7 × NbPDSrev7
NbPDS (345 bp + polyG) NbPDSfor7 × NbPDSrev8