Table 2 Oligonucleotide combinations used for amplification of NbPDS VIGS inserts
From: Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus
Construct name | Primer combination |
|---|---|
NbPDS (2046 bp) | NbPDSfor1 × NbPDSrev1 |
NbPDS (1661 bp) | NbPDSfor2 × NbPDSrev1 |
NbPDS (1304 bp) | NbPDSfor3 × NbPDSrev1 |
NbPDS (959 bp) | NbPDSfor4 × NbPDSrev1 |
NbPDS (610 bp) | NbPDSfor5 × NbPDSrev1 |
NbPDS (257 bp) | NbPDSfor6 × NbPDSrev1 |
NbPDS (345 bp) | NbPDSfor3 × NbPDSrev2 |
NbPDS (192 bp) | NbPDSfor7 × NbPDSrev3 |
NbPDS (103 bp) | NbPDSfor7 × NbPDSrev4 |
NbPDS (54 bp) | NbPDSfor8 × NbPDSrev5 |
NbPDS (385 bp) | NbPDSfor1 × NbPDSrev6 |
NbPDS (357 bp) | NbPDSfor2 × NbPDSrev7 |
NbPDS (349 bp) | NbPDSfor4 × NbPDSrev8 |
NbPDS (353 bp) | NbPDSfor5 × NbPDSrev9 |
NbPDS (2046 bp + polyA) | NbPDSfor1 × NbPDSrev6 |
NbPDS (1661 bp + polyA) | NbPDSfor2 × NbPDSrev6 |
NbPDS (1304 bp + polyA) | NbPDSfor3 × NbPDSrev6 |
NbPDS (959 bp + polyA) | NbPDSfor4 × NbPDSrev6 |
NbPDS (610 bp + polyA) | NbPDSfor5 × NbPDSrev6 |
NbPDS (257 bp + polyA) | NbPDSfor6 × NbPDSrev6 |
NbPDS (345 bp + polyA) | NbPDSfor7 × NbPDSrev7 |
NbPDS (345 bp + polyG) | NbPDSfor7 × NbPDSrev8 |