Figure 6
From: Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus
Silencing of putrescine N -methyltransferase ( PMT ) gene using cDNAs derived from the VIGS-cDNA libraries, NBREL1 and NBLEL2. (a) Position and length of NbPMT cDNA fragments of NbPDS used for silencing relative to the full-length NbPMT cDNA. Orientation of the inserts relative to the TRV coat protein is indicated by arrows. (b) Leaf nicotine levels in plants infected with TRV-NbPMT constructs as determined by HPLC. Buffer control plants received infiltration buffer only and TRV-GFP plants were infected with TRV containing a non-functional 363 bp GFP insert. Bars represent mean ± SD (n = 4).