Figure 5
From: Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus
Size distribution of cDNA inserts in two subtracted VIGS-cDNA libraries. cDNA library NBREL1 was constructed using mRNA from methyljasmonate-treated N. benthamiana roots as tester and untreated root mRNA as driver. cDNA library NBLEL2 was constructed using by mRNA pooled from methyljasmonate-treated roots as tester and untreated leaf mRNA as driver. We analyzed 150 sequenced cDNAs from NBREL1 and 77 from NBLEL2 to determine the length of each insert.