Skip to main content
Figure 2 | Plant Methods

Figure 2

From: Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus

Figure 2

Effect of cDNA insert position on the silencing of PDS in N. benthamiana leaves. (a) Position and length of NbPDS cDNA fragments used for silencing relative to the full-length NbPDS cDNA. All inserts were in the antisense orientation relative to the TRV coat protein. (b) Chlorophyll a levels in the aerial parts of plants infected with TRV-NbPDS constructs. Buffer control plants received infiltration buffer only and empty TRV plants were infected with TRV lacking a silencing insert. Chlorophyll a values for buffer, empty TRV and some constructs are also shown in Figure 1B. Bars represent mean ± SD (n = 3). (c) Photographs of representative leaves from TRV infected plants showing the extent of the photobleaching.

Back to article page