Figure 1From: Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway® TOPO vector systemSchematic from PCR gene cloning to Agrobacterium expression clones using the Gateway® cloning technology. Col E1 ori: origin of replication for E. coli. pVS1 ori: origin of replication for Agrobacteria. Kanr: kanamycin resistance gene. Cmr: chloramphenicol resistance gene. RB: T-DNA right border. LB: T-DNA left border. att L, att R, att B: recombination sites. ccdB: E. coli ccdB gene (note: the ccdB protein interferes with E. coli DNA gyrase and inhibits growth of most E. coli strains, e.g., DH5α™).Back to article page