Table 2 Tests of functionality of the newly generated constructs.
From: A modified Gateway cloning strategy for overexpressing tagged proteins in plants
REal – derived pENTR constructs | SSR – derived pDEST construct | Expression in N. benthamiana | Expression in Arabidopsis |
|---|---|---|---|
pE2n-H2A | pH2n-H2A | Yes | Yes |
pE2c-H2A | pH2c-H2A | Yes | Yes |
pE2n-H2B | pH2n-H2B | Yes | Yes |
pE2c-H2B | pH2c-H2B | Yes | Yes |
pE1c-H2B | pB1c-H2B | NT | Yes |
pE2n-UBI | pH-2n-UBI | Yes | Yes |
pE6n-UBI | pB6n-UBI | NT | Yes |
pE5n-UBI | pH5n-UBI | NT | Yes |
pE1n-UBI | pB1n-UBI | NT | Yes |
pE3n-DET1 | pK3n-DET1 | Yes | NT |
pE6n-DET1 | pB6n-DET1 | NT | No |
pE6n-DET1 | pM6n-DET1 | NT | Yes |
pE1n-DET1 | pB1n-DET1 | Yes | No |
pE2n-DDB1 | pH2n-DDB1 | Yes | NT |
pE3n-DDB1 | pK3n-DDB1 | Yes | NT |
pE2c-DDB1 | pH2c-DDB1 | Yes | NT |
pE3c-DDB1 | pK3c-DDB1 | Yes | NT |