Figure 4
From: A modified Gateway cloning strategy for overexpressing tagged proteins in plants
Transient expression in Nicotiana benthamiana tobacco leaves. A, Protein extracts (20 μg) from tobacco leaves transiently transformed with pK3nDET1 and pH2nDDB1 either alone or in combination were separated on 10% SDS-PAGE and analysed by western blotting using antibodies against HA and c-Myc as indicated. Ponceau staining is shown as loading control below each panel. B, Immunoprecipitation analysis of protein extracts (200 μg) from leaves transformed with the indicated tagged proteins with c-Myc antibody. Total extracts (30 μg) and immunoprecipitated samples were resolved on 10% SDS-PAGE and probed with α-HA antibody.