Transient expression in Nicotiana benthamiana tobacco leaves. A, Protein extracts (20 μg) from tobacco leaves transiently transformed with pK3nDET1 and pH2nDDB1 either alone or in combination were separated on 10% SDS-PAGE and analysed by western blotting using antibodies against HA and c-Myc as indicated. Ponceau staining is shown as loading control below each panel. B, Immunoprecipitation analysis of protein extracts (200 μg) from leaves transformed with the indicated tagged proteins with c-Myc antibody. Total extracts (30 μg) and immunoprecipitated samples were resolved on 10% SDS-PAGE and probed with α-HA antibody.