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Table 1 Activities of RLUC mutants selected for improved enzymatic activity.

From: Mutational optimization of the coelenterazine-dependent luciferase from Renilla

Mutations

Name

Activity ± SD

Condition

Native RLUC cDNA

none

His-RLUC

100

in vitro 1)

V267I

-

163 ± 33

in vitro

K189V

-

128 ± 16

in vitro

K189V+V267I

RLUC+

317 ± 82

in vitro

M185V+K189V+V267I

SuperRLUC

411 ± 113

in vitro

Codon-optimized RLUC cDNA (hRLUC)

none

His-hRLUC

100

in vivo 2)

K189V

-

175 ± 70

in vivo

M185V+K189V

-

425 ± 120

in vivo

M185V+K189V+V267I

SuperhRLUC

475 ± 130

in vivo

  1. 1) In vitro activites are initial luminescence values upon addition of substrate (2 μM coelenterazine). The unmutated version was set to 100.
  2. 2) In vivo activities are luminescence activities from E. coli strain BL21 without IPTG induction (2 μM coelenterazine).
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Plant Methods

ISSN: 1746-4811