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Table 1 Activities of RLUC mutants selected for improved enzymatic activity.

From: Mutational optimization of the coelenterazine-dependent luciferase from Renilla

Mutations Name Activity ± SD Condition
Native RLUC cDNA
none His-RLUC 100 in vitro 1)
V267I - 163 ± 33 in vitro
K189V - 128 ± 16 in vitro
K189V+V267I RLUC+ 317 ± 82 in vitro
M185V+K189V+V267I SuperRLUC 411 ± 113 in vitro
Codon-optimized RLUC cDNA (hRLUC)
none His-hRLUC 100 in vivo 2)
K189V - 175 ± 70 in vivo
M185V+K189V - 425 ± 120 in vivo
M185V+K189V+V267I SuperhRLUC 475 ± 130 in vivo
  1. 1) In vitro activites are initial luminescence values upon addition of substrate (2 μM coelenterazine). The unmutated version was set to 100.
  2. 2) In vivo activities are luminescence activities from E. coli strain BL21 without IPTG induction (2 μM coelenterazine).