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Table 4 A comparison of qPCR and Southern analysis results.

From: High-throughput Agrobacterium-mediated barley transformation

Plant line

qPCR copy number estimation

Number of bands obtained in Southern analysis

90-04-01

1

1

90-06-01

1

1

70-05-01

2

3

70-07-01

2

2

70-21-01

4

3

84-07-01

5

4

87-07-01

6

4

81-14-01

7

4