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Table 1 Primer and probe sequences used for quantitative real-time PCR for determining copy number of the luciferase gene.

From: High-throughput Agrobacterium-mediated barley transformation

 

Luciferase

 

Barley CONSTANS-like CO2 genea

 

Primers

LucF1

CCGCCTGAAGTCTCTGATTAAGTAC

HvCon2F1

TGCTAACCGTGTGGCATCAC

 

LucR1

GACACCTGCGTCGAAGATGTT

HvCon2R1

GGTACATAGTGCTGCTGCATCTG

Probes

LucP

6FAM-AGGCTATCAGGTGGCTCCCGCTG-TAMRA

HvCon2P

VIC-CATGAGCGTGTGCGTGTCTGCG-TAMRA

  1. aThe barley CONSTANS-like CO2 gene was used as an internal positive control.