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Figure 4 | Plant Methods

Figure 4

From: Visualizing the actin cytoskeleton in living plant cells using a photo-convertible mEos::FABD-mTn fluorescent fusion protein

Figure 4

Localized photo-activation of mEosFP::FABD-mTn along with a YFP-SKL marker targeted to peroxisomes demonstrates the feasibility of using the F-actin marker in simultaneous multicolour live-imaging schemes. The left portion of the cell was photo-converted and exhibits a visible change in coloration. The ICC-compliant standard representation of colour coding has been followed. The colour bar thus extends between absolute red (R255 G0/ff0000) to absolute green (R0 G255/00ff00) and demonstrate the spread of colours in the image. Small regions of interest sampled using the eye-dropper tool in Adobe Photoshop provided direct read-outs of colour values from the image. Note that peroxisomes (arrowheads) highlighted using YFP-SKL maintain a median (R255 G255/ffff00) value.

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Plant Methods

ISSN: 1746-4811