Photo-conversion of mEosFP-cytosolic following its transient expression in an onion epidermal cell. A.B.C. Unconverted mEosFP-Cyto visualized in green channel (500 to 525 nm) 'A'; and red channel (585 to 680 nm) 'B'. C depicts the fluorescence intensity graph observed in images A and B using the poly-line ROI (arrowheads) traced out in panel D. D.E.F. mEosFP-cyto fluorescence in green 'D' and red 'E' channels observed approximately 10 seconds after photo-conversion. F depicts the combined fluorescence intensity for D and E. Images were acquired within 10 seconds of photo-conversion. Note the shift in fluorescence intensities of red and green fluorescence between pre- and post-conversion states.