Figure 4

Arabidopsis α-tubulin is S-acylated. Subjecting Arabidopsis root whole cell lysates to the biotin switch protocol demonstrated that α-tubulin is S-acylated. Samples were treated with (Hyd+) or without (Hyd-) the thioester cleavage reagent hydroxylamine. The loading controls show that equal amounts of α-tubulin were loaded onto neutravidin beads. The lanes labelled 'S-acylation' show the levels of α-tubulin recovered from the neutravidin beads, and therefore originally S-acylated. α-tubulin has a mass of approximately 49 kDa. The results indicate that α-tubulin is S-acylated in planta.