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Table 3 Organ-enhanced TF genes

From: A community resource for high-throughput quantitative RT-PCR analysis of transcription factor gene expression in Medicago truncatula

A Root-enhanced TF genes identified by real-time RT-PCR

 

Real-time RT-PCR Expression Ratio

Affymetrix Expression Ratio

 

Accession Number

R/L

R/S

R/F

R/P

R/N

R/L

R/S

R/F

R/P

R/N

Affymetrix Chip ID

AC140721_12.1

121.5

320.1

1623.4

2676.5

64.4

161.2b

144.5b

219.2b

158.1b

99.9

Mtr.50075.1.S1_s_at

AC135101_25.1

312.2

438.0

291.6

787.0

61.3

n

n

n

n

n

n

AC140721_14.1

1204.9

751.2

956.6

6296.6

32.6

n

n

n

n

n

n

AC140031_3.1

275.4

1157.3

923.4

258916.9a

31.1

0.4

0.7

0.8

0.9

2.2

Mtr.47227.1.S1_s_at

AC140721_13.1

39.9a

179.8

614.8

935.1a

26.5

35.9

48.1b

41.2

44.2b

39.5

Mtr.50074.1.S1_at

AC140031_7.1

360.2a

830.6

1542a

6868.8

19.7

0.7b

1b

1.1b

1b

0.9b

Mtr.47229.1.S1_at

AC146574_6.1

1095.7

11.9 a

48.8

51.8

88.8

1.2b

1.1b

1.3b

1.1b

1.2b

Mtr.40781.1.S1_s_at

AC125478_13.7

1117.4

7008.0

3264.9a

7256.6

11.6

211.5b

248.8b

296.4b

228.7b

45.3

Mtr.15416.1.S1_at

AC125478_7.2

1052.3

503.3

938.4a

2754.5

11.2

n

n

n

n

n

n

AC122726_21.111

50.0

48.0

20943.9

2658.6

10.4

74.3b

76.4b

72.8b

82.8b

26.6

Mtr.15568.1.S1_s_at

B Nodule-enhanced TF genes identified by real-time RT-PCR

 

Real-time RT-PCR Expression Ratio

Affymetrix Expression Ratio

 

Accession Number

N/L

N/S

N/F

N/P

N/R

N/L

N/S

N/F

N/P

N/R

Affymetrix Chip ID

AC148816_3.2

9966.7

1826.2

6262.9

21709.1

662.7

434.6b

516.8b

455b

521.7b

551.4b

Mtr.14503.1.S1_at

AC147774_3.2

55.2 a

466.7

726.3

881.2

498.5

17.7b

16b

17.8b

19.1b

16.1b

Mtr.19554.1.S1_at

AC138056_33.241

43.9

93.2

18.3

17.3

13.1

0.9b

0.8b

0.8b

1.1b

1.1b

Mtr.17993.1.S1_at

AC124214_39.2

121.8

92.1

44.8

64.9

10.5

n

n

n

n

n

n

C Pod-enhanced TF genes identified by real-time RT-PCR

 

Real-time RT-PCR Expression Ratio

Affymetrix Expression Ratio

 

Accession Number

P/L

P/S

P/F

P/N

P/R

P/L

P/S

P/F

P/N

P/R

Affymetrix Chip ID

AC143340_4.7

154.1

44.8

173.3

180.3a

407.3

1.6b

1.8b

1.7b

1.7b

1.7b

Mtr.17931.1.S1_at

D Flower-enhanced TF genes identified by real-time RT-PCR

 

Real-time RT-PCR Expression Ratio

Affymetrix Expression Ratio

 

Accession Number

F/L

F/S

F/P

F/N

F/R

F/L

F/S

F/P

F/N

F/R

Affymetrix Chip ID

AC129092_13.1

40.3

383.3

65.1

1683.9

318.1

24.2

47.1b

38.2

45.4b

55.1b

Mtr.16432.1.S1_at

AC148485_10.1

476.2

203.7

27.2

174.6

44.5

36.5b

32.9b

22.0

34.7b

29.7b

Mtr.20392.1.S1_at

AC140915_20.1

169.5

101.1

18.6

481.0

178.7

1.4b

1.4b

1.3b

1.3b

1.6b

Mtr.51688.1.S1_at

AC141107_50.2

38.2

33.0

17.9

114.0

97.4

n

n

n

n

n

n

AC144731_15.21

64.4

44.3

33.6

61.5

16.3

0.6b

0.6b

0.8b

0.8b

0.8b

Mtr.19093.1.S1_at

AC150978_12.1

235.3

198.5

48.4

14.0

16.9

n

n

n

n

n

n

AC141107.5.61

261.1

127.8

13.3

96.4

71.5

0.9b

0.9b

0.8b

0.1b

1.1b

Mtr.51651.1.S1_at

AC157472_19.1

60.2a

92.1

13.2

61.5a

113.6

n

n

n

n

n

n

AC148527_19.141

188.4

42.1

17.3

26.5

13.0

n

n

n

n

n

n

AC144726_6.1

254.7

55.2

10.9

112.7a

754.7

59.2

73.7b

7.6

89.2

97.9b

Mtr.19024.1.S1_at

AC157488_16.1

7053.2a

141.1

18.7

81.8

10.7

n

n

n

n

n

n

AC123899_15.181

10.2

92.2

19.1

62.8

24.9

0.9b

0.5b

0.8b

0.1b

0.8b

Mtr.52015.1.S1_at

  1. A TF gene was considered organ-enhanced if transcript levels for that gene were more than 10-fold higher in one organ than in any other organ. Transcript ratios were calculated using the mean of three biological replicates for each organ. Data from qRT-PCR are compared with data for the same RNA samples obtained from Affymetrix Gene chips [38]. Affymetrix data were normalized using the Robust Multiarray Average (RMA) method, as described by [38], prior to calculation of ratios. Data in bold represent the lowest transcript ratio of the corresponding gene across all organs. n = not present on Affymetrix chip; a = Ct > 40 in two or three biological replicates of denominator organ; b = transcript called 'absent' by Affymetrix software in two or three biological replicates of denominator organ. L = leaf; S = stem; P = pod; F = flower; R = root; N = nodule.