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Figure 5 | Plant Methods

Figure 5

From: A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species

Figure 5

Amplification and melt curve graphs of LSC (rpoC1 and matK), SSC (psaC), and IR (16S) representative genes from all three Poaceae species. A. Maize B. Switchgrass C. Miscanthus. The left graph of each pair is the PCR amplification curve. The x-axis denotes the PCR cycle number and the y-axis the base-line subtracted recorded fluorescence units. The right chart illustrates the melt curve for each amplicon. The x-axis depicts the temperature in Celsius and the y-axis depicts the change in recorded fluorescence units over time.

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