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Figure 2 | Plant Methods

Figure 2

From: A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species

Figure 2

Sensitivity difference between endpoint RT-PCR and q2(RT)PCR for genes found in multiple organelles. Endpoint reveals one product for both 4.5S utilizing suboptimal primers and 16S (optimal primers) whereas the q2(RT)PCR melt curve for 4.5S clearly shows three peaks denoting three products. q2(RT)PCR melt curve for 16S verified only one product. This primer set for 23S was not analyzed by q2(RT)PCR due to the presence of multiple endpoint products. A redesign of the 23S 3' primer was accomplished and is reported in Additional File 1.

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