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Table 1 Selected reference genes for rice and corresponding primer pair information.

From: A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors

Locus identifier

Gene name

Primer sequence F/R [5'-3']

Amplicon length [bp]

Amplicon Tm [°C]

PCR efficiency

Os03g50890

Actin

CTCCCCCATGCTATCCTTCG

TGAATGAGTAACCACGCTCCG[S]

91

81

1.9

Os05g36290*

Actin1

ATCCTTGTATGCTAGCGGTCGA

ATCCAACCGGAGGATAGCATG[D]

118

80

1.9

Os01g59150*

β-Tubulin

GGAGTCACATGCTGCCTAAGGTT

TCACTGCCAGCTTACGGAGG[S]

64

80

1.96

Os06g11070

Expressed protein

AGGCTGGTCGAGGAGTCCAT

TTCTCCTCCCTAGCGAACACCT[D]

101

85

1.83

Os03g55270

TIP41-like

GTTTGGATGAACCCCGCAA

GGCAACAAGGTCAATCCGATC[S]

62

77.5

1.81

Os08g19610

Cyclophilin

CCACCATCACAGATCGGATCTT

GCGGTCAGAGCGAAAGTAGCTA[S]

65

84.2

1.73

Os03g08020*

Elongation factor 1α

GTCATTGGCCACGTCGACTC

TGTTCATCTCAGCGGCTTCC[S]

118

83.5

1.85

  1. * Primer pair recognizes all splicing variants. Location of forward and reverse primer in the same exon [S] or in different exons [D].
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Plant Methods

ISSN: 1746-4811