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Figure 3 | Plant Methods

Figure 3

From: Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae)

Figure 3

Endogenous expression levels of AqPDS and TRV1/TRV2. (a) Semi-quantitative RT-PCR of AqPDS expression for selected untreated, mock treated, treated unbleached, and treated photobleached seedlings. Seedlings had 1–2 true leaves (1–2L) or 3–5 true leaves (3–5L) at the time of treatment. Normalized values for the AqPDS/ACTIN ratios are shown for each sample. A value of 0.0 may indicate that the band was below the detection limit of the imaging system. (b) RT-PCR detection of TRV1 and TRV2 viral RNAs in selected untreated, mock treated, treated unbleached, and treated photobleached seedlings.

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Plant Methods

ISSN: 1746-4811