AqPDS silencing phenotypes. (a) Variation in AqPDS silencing phenotypes in the leaves of TRV2-AqPDS treated Aquilegia seedlings. (b) Whole seedlings exhibiting photobleaching 3–4 weeks post-treatment with TRV1 and TRV2-AqPDS. (c) Plant exhibiting strong photobleached phenotype 3–4 months post-treatment with TRV2-AqPDS. Weakly photobleached leaves represent the first leaves that arose after treatment. These leaves were maintained by the plant while later arising leaves, which showed strong photobleaching, senesced and were subsequently removed. The most recent leaves, still exhibiting strong photobleaching, are present.