Analysis of cpDNA content by qPCR of chloroplasts obtained from fractions spanning the distribution of SYTO 42 fluorescence values. (A) SYTO 42 fluorescence profile of chloroplasts from mature leaves of 43-day-old plants showing the four fractions collected by FACS. Chloroplasts were not fixed. (B) Schematic diagram of the chloroplast genome showing the location of the primer sets used for qPCR analysis. Numbers indicate distance (in kb) on the genome map  (C) qPCR analysis of cpDNA content for the four fractions described in (A). Values shown are means ± standard error for 5 or 6 replicates.