Reliable allele detection using SNP-based PCR primers containing Locked Nucleic Acid: application in genetic mapping

Table 3 PCR primers for amplification of COSII markers previously mapped to tomato (S. lycopersicum) chromosome XI.

COSII marker	Primer (5'–3')
	Forward	Reverse
C2_At5g09880	AGGGTCAAGCATATCCATGTGGAC	TCTCTTGTGCATGGCTTGTTGAGC^a
C2_At5g04590	ATCACCACAGTCCTTGCACAGGG	AGGACAAAGTGGAAAAGCTGGG^a
C2_At3g54470	TCCTGACTTTGGTTCTAAGCTTAGATCG^a	TCAAATATTAAGAAGTTGTGCTTGTCTGC

^aPrimers that were also used in combination with the respective AS SPD primer for SNP genotyping (see text; SNP genotyping). Primer source: SOL Genomics Network [30].