Figure 2
From: Function and anatomy of plant siRNA pools derived from hairpin transgenes
siRNA detection by RNase Protection Assay (RPA). Synthetic mC9 siRNA (c9si) and different sources of RNA were probed by RPA (top) using a mouse caspase 9 anti-sense probe. The positions of intact 32P-labeled probe (~200 nt), siRNAs, xylene cyanol (XC, ~60 bp) and bromophenol blue (BPB, ~15 bp) are indicated. tR (yeast tRNA); Gsi (GAPDH synthetic siRNA); c9si (single mC9 synthetic siRNA); Tc9 1 and 2 (total RNA samples from two Tc9 plants); WT (total RNA from wt plant); Dc9 (Dicer-derived mC9 siRNA); BL (body-labeled Dicer siRNA). Bands marked Δ are artifacts of the RPA as described in the text. Quantitation of Dc9si (bottom) was achieved by comparison with known amounts of synthetic siRNAs following resolution on 15% non-denaturing PAGE gels and staining with EtBr.