Figure 5From: Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAsmiRNA SYBR Green I assay. Real-time PCR amplification profiles of miR156, miR159 and miR167. The amounts of RNA used for stem-loop pulsed RT were as follows: 1, 20 ng; 2, 2 ng; 3, 200 pg; 4, 20 pg; 5, 20 ng minus-RT control; 6, water control.Back to article page