Figure 5
From: Methods for transient assay of gene function in floral tissues
Transient GUS gene expression in tobacco and petunia flowers using Agrobacterium tumefaciens infiltration. Petals of tobacco (A, B and C) and petunia (D) were stained for GUS activity 1.5 to 3 days after infiltration with A. tumefaciens strain LBA4404 harbouring either the control plasmid pART27 (A) or the 35S:IGUS construct p27IGUS (B, C and D). The petals were infiltrated while attached to the plant (A and B) or detached (C and D). Cuts were made in the petals to enable penetration of the GUS substrate.