Figure 4
From: Methods for transient assay of gene function in floral tissues
Inhibition of the function of CHS in Antirrhinum flower buds using transient RNAi based on the plasmid vector pDAH1. Petals of buds (line 522) cultured in vitro after the biolistic introduction of the control plasmid pRT99GFP (A) or the pDAH1-based plasmid pPN283 for CHS RNAi inhibition (B, C and D). (A) and (B) show buds 7 days after bombardment. (C) shows an example of inhibition in the inner epidermis, and (D) in the outer epidermis.