Figure 2

Inhibition of Rosea1 activity in Antirrhinum flower buds using transient RNAi. Petals of buds (line 522) cultured in vitro are shown 12–17 days after the biolistic introduction of the control plasmid pRT99GFP (A) or the pPN107 plasmid for Rosea1 RNAi inhibition (B, C and D). (C) and (D) show the inner and outer epidermis, respectively, of the same region of one petal. The same pattern of inhibition on both surfaces demonstrates that the silencing signal was transmitted from the bombarded outer epidermis to the inner epidermis.