Figure 4

Diagram of the process to use repeat motifs in BAC end sequence to simultaneously anchor contigs and improve the genetic map. PanelA: Two contigs from a conserved duplicated region each contain the same mapped genetic markers (black symbols), 98% similar sequences (grey symbols) or distinct sequences (white). Panel B: New markers made from BES can distinguish the regions when amplifying genomic DNA [21] more efficiently than pooled BAC DNAs of reducing complexity among the anchors described previously [11].