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Table 2 Comparison of ratiometric pHluorin an Pt-GFP when excitation ratios R(F475; F390) are taken. The optimal pH-range is calculated here from a double log-plot as shown in Figure 3

From: A novel fluorescent pH probe for expression in plants

 

GFP-species

parameter

ratiometric pHluorin

Pt-GFP

Rmax

2.65

37.4

Rmin

0.3

1.5

Rmax/Rmin

8.8

25

Rmax - Rmin

2.35

36

apparent pK (390 nm; 475 nm)

6.9

7.3

optimal pH-range

4.8 < pH < 7.6

3.8 < pH < 8.2

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