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Table 4 Comparison of camera exposure times needed for ratiometric pHluorin and Pt-GFP when excitation ratios R(F475; F390) are taken in plants under otherwise identical settings.

From: A novel fluorescent pH probe for expression in plants

 

GFP-species

parameter

ratiometric pHluorin

Pt-GFP

390 nm

180

300

475 nm

150

100