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Figure 4 | Plant Methods

Figure 4

From: A novel fluorescent pH probe for expression in plants

Figure 4

pH-stability of Pt-GFP (A) and ratiometric pHluorin (B). Agarose beads decorated with GFP and sandwiched between sheets of cellophane were dialysed against buffers of different pH as indicated by the top bars of the graphs and fluorescence (Fem = 535 ± 25 nm) was recorded with a fluorescence microscope. (Buffer composition: 50 mM Hepes, 50 mM Mes, 200 mM NaCl adjusted with NaOH or HCl to the desired pH).

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