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Figure 1 | Plant Methods

Figure 1

From: Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants

Figure 1

Diagrammatic presentation of the tetra-primer ARMS-PCR method for SNP identification (Redrawn from [58]). Two allele-specific amplicons are generated using two pairs of primers, one pair (P1 and P4) producing an amplicon representing the G allele and the other pair (P2 and P3) producing an amplicon representing the A allele. By positioning the two outer primers (P1 and P2) at different distances from the polymorphic nucleotide, the two allele-specific amplicons differ in length, allowing them to be discriminated by gel electrophoresis.

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Plant Methods

ISSN: 1746-4811