Development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a BAC minimum tile path

Table 3 Proportion of genes in different organisms present as either singletons or in paralogous families.

Species	No of genes*	Unique gene families containing
		1	2	3	4	5	> 5 member
H. influenzae	1,587	88.8%	6.8%	2.3%	0.7%	0.0%	1.4%
S. cerevisiae	5,105	71.4%	13.8%	3.5%	2.2%	0.7%	8.4%
D. melanogaster	10,736	72.5%	8.5%	3.4%	1.9%	1.6%	12.1%
C. elegans	14,177	55.2%	12.0%	4.5%	2.7%	1.6%	24.0%
Arabidopsis	11,601	35.0%	12.5%	7.0%	4.4%	3.6%	37.4%
G.max (soybean)	201	35.0%	25.0 %	10.4%	10.4%	4.5%	15%

* The number of genes in the genomes of Haemophilus influenzae, S. cerevisiae, Drosophila, C. elegans, Arabidopsis and Glycine max that were present either as singletons or in gene families with two or more members were listed. To be grouped in a gene family, two genes had to show similarity exceeding a BLASTP value E,10 -20 and a FASTA alignment over at least 80%of the protein length or hybridize at 65 C and 0.1 M ionic strength (Tm -25). In column 1, the number of genes that were unique plus the number of gene families were listed. Columns 2 to 6 give the percentage of genes present as singletons or in gene families of n members (from TAIR 2000).

ISSN: 1746-4811